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Title   흑색종 세포주의 증식 및 세포주기 조절인자에 고려인삼 지용성 성분이 미치는 영향 ( Effects of Lipid Soloble Components of Korean Red Ginseng on Proliferation and Cell Cycle Regulation Factors in Human Melanoma Cell Lines )
Publicationinfo   1997 Jan; 029(06): 965-977.
Key_word   Melanoma, Korean red ginseng, p21WAF1, p27Kip1, Rb
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Abstract   Purpose: Growth inhibitory effects of lipid soluble components of the Korean red ginseng and the antineoplastic mechanism against human melanoma cell lines were investigated. To examine molecular mechanism of growth inhibitory effects of GX-PE, we analyzed the effect of GX-PE on cell cycle progression and expression of cell cycle regulatory factors such as retinoblastoma gene product (Rb), p27Kip1, p21WAF1, cdk2, cdk4 and cyclin D1 which are known to regulate cell cycle progression. Materials and Methods: Petraleum ether extract of the Korean red ginseng (GX-PE) was added to cultures of three human melanoma cell lines, SK-MEL-1, SK-MEL-2, and SK-MEL-5. Proliferation was measured by H-thymidine incorporation assay. Cell cycle and expression of cell cycle regulatory factors were analyzed by flow cytometry and Western blotting, respectively. Results: Growth of melanoma cells was inhibited by GX-PE in proportion to the concentration. GX-PE significantly inhibited cell cycle progression at Gl phase. GX-PE increased expression of negative cell cycle regulators, i.e., p27Kip1 in SK-MEL-2 and p21WAF1 and Rb in SK-MEL-1. Conclusion: These results suggest that GX-PE inhibits proliferation of melanoma cells at a Gl-S transition point of the cell cycle. The effect of GX-PE is most likely due to induction of negative cell cycle regulatory factors.
저 자   김혜정(Hye Jung Kim),노주영(Joo Young Noh),최종욱(Jong Wook Choi),박설희(Sul Hee Park),김인선(In Sun Kim),손정원(Jung Won Son),류총근(Chong Geun Ryoo)